NotI Recognition Site
NotI at a glance
5' GC|GGCCGC 3' 3' CGCCGG|CG 5'
| Recognition site | 5'-GCGGCCGC-3' |
| Cut | GC^GGCCGC |
| Ends | a 4-base 5' overhang (GGCC) |
| Expected frequency | about once every 65,536 bp in random DNA |
| Compatible ends | EagI |
How to find NotI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. NotI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each NotI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does NotI cut?
NotI recognizes GCGGCCGC (written 5' to 3') and cuts the top strand at GC^GGCCGC.
Does NotI leave sticky or blunt ends?
Sticky ends: a 4-base 5' overhang (GGCC).
Which enzymes leave ends compatible with NotI?
EagI. Their overhangs are the same, so the ends can be ligated to each other.
How often does NotI cut?
A site like GCGGCCGC turns up about once every 65,536 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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