AscI Recognition Site
AscI at a glance
5' GG|CGCGCC 3' 3' CCGCGC|GG 5'
| Recognition site | 5'-GGCGCGCC-3' |
| Cut | GG^CGCGCC |
| Ends | a 4-base 5' overhang (CGCG) |
| Expected frequency | about once every 65,536 bp in random DNA |
| Compatible ends | BssHII, MluI |
How to find AscI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. AscI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each AscI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does AscI cut?
AscI recognizes GGCGCGCC (written 5' to 3') and cuts the top strand at GG^CGCGCC.
Does AscI leave sticky or blunt ends?
Sticky ends: a 4-base 5' overhang (CGCG).
Which enzymes leave ends compatible with AscI?
BssHII and MluI. Their overhangs are the same, so the ends can be ligated to each other.
How often does AscI cut?
A site like GGCGCGCC turns up about once every 65,536 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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