PmeI Recognition Site
PmeI at a glance
5' GTTT|AAAC 3' 3' CAAA|TTTG 5'
| Recognition site | 5'-GTTTAAAC-3' |
| Cut | GTTT^AAAC |
| Ends | blunt ends |
| Expected frequency | about once every 65,536 bp in random DNA |
How to find PmeI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. PmeI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each PmeI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does PmeI cut?
PmeI recognizes GTTTAAAC (written 5' to 3') and cuts the top strand at GTTT^AAAC.
Does PmeI leave sticky or blunt ends?
Blunt ends, with no overhang.
How often does PmeI cut?
A site like GTTTAAAC turns up about once every 65,536 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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