Toolyard

Reverse Translate a Protein Alignment

How to reverse translate a protein alignment

  1. Paste a protein alignment in FASTA format, with gaps written as -.
  2. Choose the genetic code.
  3. Each column becomes one degenerate codon that codes for every amino acid in that column.

Frequently asked questions

What is it for?

Designing degenerate primers that bind the gene of every protein in the alignment. Pick a stretch with little degeneracy for the primer.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.

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