Restriction Summary

How to find which enzymes cut your DNA

1. Paste a DNA sequence, as plain text or FASTA.
2. Choose which enzymes to show: all that cut, single or double cutters, or those that do not cut. Tick Circular DNA for a plasmid.
3. Each enzyme is listed with its site, the number of cuts, the cut positions and the fragment sizes.

Frequently asked questions

Which enzymes are included?

A set of 65 common commercial enzymes with palindromic sites, such as EcoRI, BamHI, HindIII, NotI and XhoI.

Where is the cut position?

The position of the last base before the cut on the top strand.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.