Mutate for Digest

Coding DNA sequence

Reading frameGenetic codeMost changes per site

Output

How to add a restriction site with silent mutations

Paste a coding DNA sequence.
Choose the reading frame, the genetic code and whether one or two base changes are allowed per site.
Every site that can be created without changing the protein is listed with the enzyme, position, changes and new sequence.

Frequently asked questions

Why are some enzymes missing?

Enzymes that already cut the sequence are left out, since a new site would not be unique.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.

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