Toolyard

PCR Products

How to predict PCR products

  1. Paste the template DNA.
  2. Type the forward and reverse primers, both 5' to 3', as you would order them.
  3. Each product is listed as FASTA with its length and position. Tick Circular template for a plasmid.

Frequently asked questions

Do the primers have to match exactly?

Only the 3' end has to match; set how many bases. The 5' end can differ, so primers with added restriction sites or tags work, and the product includes them.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.

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