PCR Products

Template DNA

Forward primer (5' to 3')Reverse primer (5' to 3')Bases at the 3' end that must match Circular template

Output

How to predict PCR products

Paste the template DNA.
Type the forward and reverse primers, both 5' to 3', as you would order them.
Each product is listed as FASTA with its length and position. Tick Circular template for a plasmid.

Frequently asked questions

Do the primers have to match exactly?

Only the 3' end has to match; set how many bases. The 5' end can differ, so primers with added restriction sites or tags work, and the product includes them.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.

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Guides

How to Design and Check PCR Primers How to Design qPCR Primers