MfeI Recognition Site
MfeI at a glance
5' C|AATTG 3' 3' GTTAA|C 5'
| Recognition site | 5'-CAATTG-3' |
| Cut | C^AATTG |
| Ends | a 4-base 5' overhang (AATT) |
| Expected frequency | about once every 4,096 bp in random DNA |
| Compatible ends | EcoRI |
How to find MfeI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. MfeI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each MfeI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does MfeI cut?
MfeI recognizes CAATTG (written 5' to 3') and cuts the top strand at C^AATTG.
Does MfeI leave sticky or blunt ends?
Sticky ends: a 4-base 5' overhang (AATT).
Which enzymes leave ends compatible with MfeI?
EcoRI. Their overhangs are the same, so the ends can be ligated to each other.
How often does MfeI cut?
A site like CAATTG turns up about once every 4,096 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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