EagI Recognition Site
EagI at a glance
5' C|GGCCG 3' 3' GCCGG|C 5'
| Recognition site | 5'-CGGCCG-3' |
| Cut | C^GGCCG |
| Ends | a 4-base 5' overhang (GGCC) |
| Expected frequency | about once every 4,096 bp in random DNA |
| Compatible ends | NotI |
How to find EagI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. EagI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each EagI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does EagI cut?
EagI recognizes CGGCCG (written 5' to 3') and cuts the top strand at C^GGCCG.
Does EagI leave sticky or blunt ends?
Sticky ends: a 4-base 5' overhang (GGCC).
Which enzymes leave ends compatible with EagI?
NotI. Their overhangs are the same, so the ends can be ligated to each other.
How often does EagI cut?
A site like CGGCCG turns up about once every 4,096 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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