FseI Recognition Site
FseI at a glance
5' GGCCGG|CC 3' 3' CC|GGCCGG 5'
| Recognition site | 5'-GGCCGGCC-3' |
| Cut | GGCCGG^CC |
| Ends | a 4-base 3' overhang (CCGG) |
| Expected frequency | about once every 65,536 bp in random DNA |
How to find FseI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. FseI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each FseI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does FseI cut?
FseI recognizes GGCCGGCC (written 5' to 3') and cuts the top strand at GGCCGG^CC.
Does FseI leave sticky or blunt ends?
Sticky ends: a 4-base 3' overhang (CCGG).
How often does FseI cut?
A site like GGCCGGCC turns up about once every 65,536 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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