Reverse Complement
How to get the reverse complement of DNA
- Paste one or more DNA or RNA sequences, as plain text or FASTA.
- Choose reverse complement, reverse or complement. The output updates as you type.
- Copy output or Download saves the result.
Frequently asked questions
What happens to ambiguity codes?
IUPAC codes are complemented too: R becomes Y, K becomes M, B becomes V, and N, S and W stay the same. Lowercase letters stay lowercase.
What is the difference between reverse, complement and reverse complement?
Reverse writes the sequence backwards. Complement swaps each base for its partner (A with T, C with G). Reverse complement does both, which gives the other strand read 5' to 3'.
Is my sequence uploaded?
No. Everything runs in your browser, so your sequences never leave your device.
Can I see how the sequence folds?
The DNA and RNA Folding Viewer predicts the lowest-energy pairing of a single strand and draws its stems and loops in 3D, with the free energy and melting temperature.
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