Toolyard

Split FASTA Sequence

How to split a sequence into fragments

  1. Paste a DNA or protein sequence, or several FASTA records.
  2. Set the fragment length and, if you want fragments to share letters, the overlap.
  3. Each fragment becomes its own FASTA record with its number and position in the title.

Frequently asked questions

What does overlap do?

With a fragment length of 100 and an overlap of 20, the fragments cover 1..100, 81..180, 161..260 and so on. The overlap must be shorter than the fragment length.

Is my sequence uploaded?

No. Everything runs in your browser, so your sequences never leave your device.

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