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SwaI Recognition Site


SwaI at a glance

5' ATTT|AAAT 3'
3' TAAA|TTTA 5'
Recognition site5'-ATTTAAAT-3'
CutATTT^AAAT
Endsblunt ends
Expected frequencyabout once every 65,536 bp in random DNA

How to find SwaI sites in a sequence

  1. Paste a DNA sequence or FASTA record into the box above. SwaI is already entered under Only these enzymes.
  2. Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
  3. The map marks each SwaI cut with its position. Copy or download the output below it.

Frequently asked questions

What sequence does SwaI cut?

SwaI recognizes ATTTAAAT (written 5' to 3') and cuts the top strand at ATTT^AAAT.

Does SwaI leave sticky or blunt ends?

Blunt ends, with no overhang.

How often does SwaI cut?

A site like ATTTAAAT turns up about once every 65,536 bp in random DNA. Real sequences vary, so check yours above.

Restriction enzymes

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