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EcoRV Recognition Site


EcoRV at a glance

5' GAT|ATC 3'
3' CTA|TAG 5'
Recognition site5'-GATATC-3'
CutGAT^ATC
Endsblunt ends
Expected frequencyabout once every 4,096 bp in random DNA

How to find EcoRV sites in a sequence

  1. Paste a DNA sequence or FASTA record into the box above. EcoRV is already entered under Only these enzymes.
  2. Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
  3. The map marks each EcoRV cut with its position. Copy or download the output below it.

Frequently asked questions

What sequence does EcoRV cut?

EcoRV recognizes GATATC (written 5' to 3') and cuts the top strand at GAT^ATC.

Does EcoRV leave sticky or blunt ends?

Blunt ends, with no overhang.

How often does EcoRV cut?

A site like GATATC turns up about once every 4,096 bp in random DNA. Real sequences vary, so check yours above.

Restriction enzymes

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