HinfI Recognition Site
HinfI at a glance
5' G|ANTC 3' 3' CTNA|G 5'
| Recognition site | 5'-GANTC-3' |
| Cut | G^ANTC |
| Ends | a 3-base 5' overhang (ANT) |
| Expected frequency | about once every 256 bp in random DNA |
How to find HinfI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. HinfI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each HinfI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does HinfI cut?
HinfI recognizes GANTC (written 5' to 3') and cuts the top strand at G^ANTC.
Does HinfI leave sticky or blunt ends?
Sticky ends: a 3-base 5' overhang (ANT).
How often does HinfI cut?
A site like GANTC turns up about once every 256 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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