MluI Recognition Site
MluI at a glance
5' A|CGCGT 3' 3' TGCGC|A 5'
| Recognition site | 5'-ACGCGT-3' |
| Cut | A^CGCGT |
| Ends | a 4-base 5' overhang (CGCG) |
| Expected frequency | about once every 4,096 bp in random DNA |
| Compatible ends | AscI, BssHII |
How to find MluI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. MluI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each MluI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does MluI cut?
MluI recognizes ACGCGT (written 5' to 3') and cuts the top strand at A^CGCGT.
Does MluI leave sticky or blunt ends?
Sticky ends: a 4-base 5' overhang (CGCG).
Which enzymes leave ends compatible with MluI?
AscI and BssHII. Their overhangs are the same, so the ends can be ligated to each other.
How often does MluI cut?
A site like ACGCGT turns up about once every 4,096 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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