DraI Recognition Site
DraI at a glance
5' TTT|AAA 3' 3' AAA|TTT 5'
| Recognition site | 5'-TTTAAA-3' |
| Cut | TTT^AAA |
| Ends | blunt ends |
| Expected frequency | about once every 4,096 bp in random DNA |
How to find DraI sites in a sequence
- Paste a DNA sequence or FASTA record into the box above. DraI is already entered under Only these enzymes.
- Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
- The map marks each DraI cut with its position. Copy or download the output below it.
Frequently asked questions
What sequence does DraI cut?
DraI recognizes TTTAAA (written 5' to 3') and cuts the top strand at TTT^AAA.
Does DraI leave sticky or blunt ends?
Blunt ends, with no overhang.
How often does DraI cut?
A site like TTTAAA turns up about once every 4,096 bp in random DNA. Real sequences vary, so check yours above.
Restriction enzymes
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