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HincII Recognition Site


HincII at a glance

5' GTY|RAC 3'
3' CAR|YTG 5'
Recognition site5'-GTYRAC-3'
CutGTY^RAC
Endsblunt ends
Expected frequencyabout once every 1,024 bp in random DNA

How to find HincII sites in a sequence

  1. Paste a DNA sequence or FASTA record into the box above. HincII is already entered under Only these enzymes.
  2. Tick Circular DNA for a plasmid, so a site that spans the end and the start is found.
  3. The map marks each HincII cut with its position. Copy or download the output below it.

Frequently asked questions

What sequence does HincII cut?

HincII recognizes GTYRAC (written 5' to 3') and cuts the top strand at GTY^RAC.

Does HincII leave sticky or blunt ends?

Blunt ends, with no overhang.

How often does HincII cut?

A site like GTYRAC turns up about once every 1,024 bp in random DNA. Real sequences vary, so check yours above.

Restriction enzymes

More DNA and protein sequence tools

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