Oligo Resuspension Calculator

How to resuspend an oligo or primer

1. Type the amount of oligo in the tube in nmol, from the tube label or the data sheet.
2. Type the stock concentration you want, usually 100 µM.
3. Add the volume of water or TE shown. To make a working solution, type its concentration and volume to see how much stock and water or TE to mix.

Frequently asked questions

What is the formula?

Volume in µL = nmol × 1,000 ÷ concentration in µM. For a 100 µM stock that is 10 µL per nmol.

Water or TE?

Low-EDTA TE (10 mM Tris, 0.1 mM EDTA, pH 8) keeps oligos stable longer in storage than water, and does not upset most PCR reactions at working dilutions.