Cell Seeding Calculator

How to calculate cell seeding volume

1. Choose the plate or flask, or Other to type its growth area, and type how many wells or flasks to seed.
2. Type the seeding density in cells per cm² and the concentration of your cell suspension from a cell count.
3. The volume of cell suspension appears, with the medium to add, the cells per well and the total cells.

Frequently asked questions

What growth areas are used?

Typical values: 0.32 cm² for a 96-well plate, 0.95 for 48, 1.9 for 24, 3.8 for 12 and 9.5 for 6 wells; 25, 75 and 175 cm² for T25, T75 and T175 flasks. Areas vary a little between makers, so choose Other to type the exact one from your plate's data sheet.

How do I mix it?

Mix the cell suspension volume shown into the medium to add, then split the mix evenly across the wells or flasks. Making about 10% extra allows for pipetting losses.