Ligation Calculator

How to calculate insert for a ligation

1. Type the vector length and amount in ng, and the insert length.
2. Type the insert to vector molar ratio, such as 3 for 3:1.
3. The mass of insert to add appears, and the volume too if you type the insert concentration.

Frequently asked questions

What ratio should I use?

3:1 insert to vector is a common start for sticky ends. Try 1:1 to 10:1 if it does not work, and higher ratios for short inserts.

What is the formula?

Insert (ng) ≈ ratio × vector (ng) × insert length / vector length.