Serial Dilution Calculator

How to plan a serial dilution

1. Type the starting concentration, the dilution factor per step and the number of dilutions.
2. Type the final volume you want in each tube.
3. The table lists each tube with its concentration, how much to transfer from the tube before, and how much diluent to add.

Frequently asked questions

How much do I transfer for a 1:10 series?

One tenth of the final volume: for 1 mL in each tube, transfer 100 µL into 900 µL of diluent.

What is the concentration after several steps?

The starting concentration divided by the dilution factor once for each step. 1 M diluted 1:10 three times is 1 mM.

Should I change tips between tubes?

Yes. Mix each tube well and use a fresh tip for each transfer, or carry-over makes the later tubes too concentrated.